Not only is it OK to be a little vague, its absolutely necessary, otherwise you re claiming more knowledge than you actually have.
Take 1 mL of that and put it in 49 mLs of water.
First of all, we re going to use the Viable Plate Count method - the only method that can tell a live cell from a dead one for a rundown on counting methods, look here.Frantically he considered derivatives, limit theorems, integrals and natural logarithms, but they all seemed a bit, well, complicated, given that dinner nero 9 reloaded 126.96.36.199 serial was to be served in six minutes and his mother was out adding a fifth spoon to every place setting.But for what it s worth, the dilution factor for 1 mL stock 99 mL water is: amount transferred / total amount amount transferred / amount transferred amount water added 1 / 1 99 1/100.01.Let s imagine that we have a sample with exactly 42 million cells per.So, when we count the caffeine molecules in a cup of wimpy coffee, we know we got 1/50th of what was in a cup of the original brew - or in other words, there was 50 times as much in a cup of the original.We need to get plate counts between 30 and 300, and we think that the concentration in his blood might be as high as 500 per mL, but it could be much lower.Then the next day, we decide which plate looks like the most reasonable for counting.In fact, even with our absolutely flawless technique, the best we can say is that there are between 41,500,000 and 42,500,000 cells.Very easily while we re doing our dilutions, we just keep plating each intermediate step.
Well, that s where the technique comes.
Notice that I am being a little cavalier with the numbers here.
I say I use about 4 bags a day, and multiply by about one hundred million.
If the dilution factor is in the form of a fraction, flip the fraction.e., 1/50 becomes multiply by 50/1.The prince s sister dilutes the soup Just then his younger sister entered the kitchen and saw his miserable face.I need a hint :Your dilution factor is another hint :Divide final dilution with your dilution factor to get initial dilution.Nope, can t do it, this is too much to count 0,050,000 to 41,050,000 /- 50,000.11 still great sorry, still too hard to count 1:1,000,000 42 41,500,000 to 41,500,000 /- 500,000.1 not too bad easy to count,000,000 4 35,000,000 to 45,000,000 /-.But the exact same thing would happen if our sample started with 183,001 cells.Hint: this applet previews the module on bacterial growth rates a bit.Given the following series of dilutions, what is the total dilution factor.So we re stuck with a viable plate count.